Characterization of polymorphic microsatellite loci for the spiny lobster Panulirus spp. and their utility to be applied to other Panulirus lobsters

Dao, Hoc Tan, Todd, Erica V., and Jerry, Dean R. (2013) Characterization of polymorphic microsatellite loci for the spiny lobster Panulirus spp. and their utility to be applied to other Panulirus lobsters. Conservation Genetics Resources, 5 (1). pp. 43-46.

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DOI: 10.1007/s12686-012-9728-0

View at Publisher Website: http://dx.doi.org/10.1007/s12686-012-972...

Abstract

The ornate spiny lobster Panulirus ornatus is a commercially important fishery and aquaculture species throughout south-east Asia and Australia. Thirty microsatellite markers were developed for P. ornatus of which 15 loci were consistently amplified and exhibited adequate levels of polymorphism for parentage and population genetic applications. Number of alleles observed per locus ranged from 2 to 23 (mean NA = 9.1 ± 1.4). Observed and expected heterozygosities ranged from 0.03–0.98 (HO mean = 0.61 ± 0.06) to 0.03–0.94 (HE mean = 0.66 ± 0.06), respectively. Microsatellite markers were also tested in three related Panulirus species, P. homarus, P. versicolor, and P. argus, with varying levels of amplification success and polymorphism exhibited.


Item ID:	23150
Item Type:	Article (Research - C1)
ISSN:	1877-7260
Keywords:	Crustacea, microsatellite, Panulirus, spiny lobster, 454 sequencing
Date Deposited:	28 Aug 2012 23:42
FoR Codes:	07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 60% 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070402 Aquatic Ecosystem Studies and Stock Assessment @ 40%
SEO Codes:	83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8302 Fisheries - Wild Caught > 830202 Wild Caught Crustaceans (excl. Rock Lobster and Prawns) @ 100%
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