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Abstract

Golgi membranes and Golgi-derived vesicles are associated with multiple cytoskeletal proteins and motors, the diversity and distribution of which have not yet been defined. Carrier vesicles were separated from Golgi membranes, using an in vitro budding assay, and different populations of vesicles were separated using sucrose density gradients. Three main populations of vesicles labeled with beta-COP, gamma-adaptin, or p200/myosin II were separated and analyzed for the presence of actin/actin-binding proteins, beta-Actin was bound to Golgi cisternae and to all populations of newly budded vesicles. Centractin was selectively associated with vesicles co-distributing with beta-COP-vesicles, while p200/myosin II (non-muscle myosin IIA) and non-muscle myosin IIB were found on different vesicle populations. Isoforms of the Tm5 tropomyosins were found on selected Golgi-derived vesicles, while other Tm isoforms did not colocalize with Tm5 indicating the association of specialized actin filaments with Golgi-derived vesicles. Golgi-derived vesicles were shown to bind to F-actin polymerized from cytosol with Jasplakinolide. Thus, newly budded, coated vesicles derived from Golgi membranes can bind to actin and are customized for differential interactions with microfilaments by the presence of selective arrays of actin-binding proteins.

Item ID:	1932
Item Type:	Article (Research - C1)
ISSN:	1083-351X
Keywords:	myosin-II, coated vesicles, tropomyosin isoforms, transport vesicles, subcellular-localization, Saccharomyces cerevisiae, transmembrane protein, vesicular transport, endocytic pathway, epithelial cells
Additional Information:	Reproduced with permission from American Society for Biochemistry and Molecular Biology.
Date Deposited:	30 Jul 2008
FoR Codes:	06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology @ 0%
Downloads:	Total: 12
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