Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)

Waycott, Michelle, Jones, Brendan L., Van Dijk, Jent Kornelis, Robson, Heather L.A., and Calladine, Ainsley (2011) Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae). American Journal of Botany, 98 (4). e81-e83.

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DOI: 10.3732/ajb.1000356

View at Publisher Website: http://dx.doi.org/10.3732/ajb.1000356

Abstract

Premise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified.

Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14.

Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.


Item ID:	18310
Item Type:	Article (Research - C1)
ISSN:	1537-2197
Keywords:	microsatellite; polyploidy; Solanum centrale
Date Deposited:	26 Sep 2011 05:20
FoR Codes:	06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060703 Plant Developmental and Reproductive Biology @ 10% 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060207 Population Ecology @ 30% 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 60%
SEO Codes:	96 ENVIRONMENT > 9605 Ecosystem Assessment and Management > 960504 Ecosystem Assessment and Management of Farmland, Arable Cropland and Permanent Cropland Environments @ 100%
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