Squires, R.A. (2005) Initial experiences with consensus primer PCR for detection of animal herpesviruses. New Zealand Veterinary Journal, 53 (1). p. 94.

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Abstract

Consensus primer polymerase chain reaction (CP-PCR) is a useful technique for the detection and partial characterisation of novel viruses of veterinary importance. CP-PCR differs from conventional PCR, in that the upstream and downstream primers each consist of a mixture of slightly different oligonucleotides, rather than a single, defined sequence. As used in virus discovery, CP-PCR amplifies a segment of the genome of all members of a particular virus group. Conserved virus genes, e.g. those encoding enzymes, are the most suitable targets when designing these strategies. We used a previously validated CP-PCR method to amplify herpesviral DNA from the left conjunctival sac of a farmed red deer (Cervus elaphus) with keratitis. Sequencing of the CP-PCR product revealed 97% homology to a 174-base pair segment of a novel rhadinovirus of elk (Alces alces) (GenBank AY237365.1). An attempt to isolate a herpesvirus from the deer's conjunctival sac in tissue culture was unsuccessful, as was PCR using conventional primers specific for Cervid herpesvirus 1. This case exemplifies the potential value of CP-PCR for detection of novel herpesviruses.

Item ID:	18275
Item Type:	Article (Abstract)
ISSN:	1176-0710
Date Deposited:	21 Nov 2011 00:32
FoR Codes:	07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070706 Veterinary Medicine @ 100%
SEO Codes:	86 MANUFACTURING > 8609 Veterinary Pharmaceutical Products > 860902 Veterinary Diagnostics @ 100%
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