Influence of enzymatic maceration on the microstructure and microhardness of compact bone

Yin, Ling, Venkatesan, Sudharshan, Kalyanasundaram, Shankar, and Qin, Qing-Hua (2010) Influence of enzymatic maceration on the microstructure and microhardness of compact bone. Biomedical Materials, 5 (1). 015006. pp. 1-6.

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Abstract

The cleaning of fresh bones to remove their soft tissues while maintaining their structural integrity is a basic and essential part of bone studies. The primary issue is how the cleaning process influences bone microstructures and mechanical properties. We cleaned fresh lamb femurs using enzymatic maceration in comparison with water maceration at room temperature. The microstructures of these compact bones were examined using scanning electron microscopy (SEM) and their porosities were quantified using image processing software. The bone microhardness was measured using a Vickers indentation tester for studying the mechanical properties. The results show that enzymatic maceration of compact bone resulted in a significant microhardness reduction in comparison with water maceration. However, enzymatic maceration did not cause any significant change of porosity in bone structures.

Item ID: 16094
Item Type: Article (Research - C1)
ISSN: 1748-6041
Date Deposited: 14 Apr 2011 06:06
FoR Codes: 09 ENGINEERING > 0913 Mechanical Engineering > 091308 Solid Mechanics @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920118 Surgical Methods and Procedures @ 100%
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