Vukomanovic, Dragic, McLaughlin, Brian, Rahman, Mona N., Vlahakis, Jason Z., Roman, Gheorghe, Dercho, Ryan A., Kinobe, Robert, Hum, Maaike, Brien, James F., Jia, Zongchao, Szarek, Walter A., and Nakatsu, Kanji (2010) Recombinant truncated and microsomal heme oxygenase-1 and -2: differential sensitivity to inhibitors. Canadian Journal of Physiology and Pharmacology, 88 (4). pp. 480-486.

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Abstract

Recombinant truncated forms of heme oxygenase-1 and -2 (HO-1 and HO-2) were compared with their crude microsomal counterparts from brain and spleen tissue of adult male rats with respect to their inhibition by azole-based, nonporphyrin HO inhibitors. The drugs tested were an imidazole-alcohol, an imidazole-dioxolane, and a triazole-ketone. Both the recombinant and crude forms of HO-2 were similarly inhibited by the 3 drugs. The crude microsomal spleen form of HO-1 was more susceptible to inhibition than was the truncated recombinant form. This difference is attributed to the extra amino acids in the full-length enzyme. These observations may be relevant in the design of drugs as inhibitors of HO and other membrane proteins.

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