Ultrasensitive detection of antibodies using a new Tus-Ter-lock immunoPCR system
Morin, Isabelle, Dixon, Nicholas E., and Schaeffer, Patrick M. (2010) Ultrasensitive detection of antibodies using a new Tus-Ter-lock immunoPCR system. Molecular BioSystems, 6 (7). pp. 1173-1175.
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DOI: 10.1039/C002163F
View at Publisher Website: http://dx.doi.org/10.1039/C002163F
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Abstract
A system consisting of a protein LG coated surface for the capture of mammalian antibodies (target), and an antigen fused to Tus and stoichiometrically linked to a DNA template via the Tus-Ter-lock sequence allowed the ultrasensitive detection of 5.5 attomol of target by real-time immunoPCR in complex media.
| Item ID: | 15634 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1742-2051 |
| Date Deposited: | 29 Mar 2011 23:04 |
| FoR Codes: | 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060101 Analytical Biochemistry @ 100% |
| SEO Codes: | 92 HEALTH > 9202 Health and Support Services > 920203 Diagnostic Methods @ 100% |
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