Absolute quantification method and validation of airborne snow crab allergen tropomyosin using tandem mass spectrometry

Rahman, Anas M. Abdel, Lopata, Andreas L., Randell, Edward W., and Helleur, Robert J. (2010) Absolute quantification method and validation of airborne snow crab allergen tropomyosin using tandem mass spectrometry. Analytica Chimica Acta, 681 (1-2). pp. 49-55.

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Abstract

Measuring the levels of the major airborne allergens of snow crab in the workplace is very important in studying the prevalence of crab asthma in workers. Previously, snow crab tropomyosin (SCTM) was identified as the major aeroallergen in crab plants and a unique signature peptide was identified for this protein. The present study advances our knowledge on aeroallergens by developing a method of quantification of airborne SCTM by using isotope dilution mass spectrometry. Liquid chromatography tandem mass spectrometry was developed for separation and analysis of the signature peptides. The tryptic digestion conditions were optimized to accomplish complete digestion. The validity of the method was studied using international conference on harmonization protocol, Where 2–9% for CV (precision) and 101–110% for accuracy, at three different levels of quality control. Recovery of the spiked protein from PTFE and TopTip filters was measured to be 99% and 96%, respectively. To further demonstrate the applicability and the validity of the method for real samples, 45 kg of whole snow crab were processed in an enclosed (simulated) crab processing line and air samples were collected. The levels of SCTM ranged between 0.36–3.92 μg m−3 and 1.70–2.31 μg m−3 for butchering and cooking stations, respectively.

Item ID: 15240
Item Type: Article (Research - C1)
ISSN: 1873-4324
Keywords: snow crab tropomyosin; quantification; validation; airborne allergen; mass spectrometry; multi reaction monitoring; isotopic dilution
Date Deposited: 27 Jan 2011 23:44
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 100%
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