Intranuclear bacilliform virus and hepatopancreatic parvovirus (PmergDNV) in the mud crab Scylla serrata (Forskal) of Australia

Owens, L., Liessmann, L., La Fauce, K., Nyguyen, T., and Zeng, C. (2010) Intranuclear bacilliform virus and hepatopancreatic parvovirus (PmergDNV) in the mud crab Scylla serrata (Forskal) of Australia. Aquaculture, 310 (1-2). pp. 47-51.

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Abstract

The culture of mud crabs Scylla serrata (Forskal) is largely experimental in Australia but some operators are producing marketable quantities. In an effort to understand the bottlenecks to production in hatcheries, a number of disease studies were initiated. In one study, basophilic to particularly magenta, intranuclear inclusions consistent with intranuclear bacilliform virus were discovered in the hepatopancreas of ~ 9% of wild broodstock and ~ 13% of batches of their progeny larvae. The inclusions were highly focused in hepatopancreatic tubules with 13-42% of tubules infected.

Concurrently, using quantitative real-time Taqman PCR, ~ 74% of haemolymph samples of adults and 50% of batches of larvae were positive for Penaeus merguiensis Densovirus (PmergDNV) with copy numbers ranging from 6 × 102 to 1.5 × 105. Sequencing of 2475 base pairs of viral genome confirmed that the virus was 99% similar to PmergDNV. This is the first record of one of the members of the hepatopancreatic parvovirus group of viruses to be found outside penaeid prawns. This study demonstrates that there are at least two viruses circulating in mud crab populations in northern Australia that might impact on hatchery production.

Item ID: 15121
Item Type: Article (Research - C1)
ISSN: 1873-5622
Keywords: mud crab, Scylla serrata, intranuclear bacilliform virus, TaqMan PCR, hepatopancreatic parvovirus, PmergDNV
Date Deposited: 21 Dec 2010 01:47
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070404 Fish Pests and Diseases @ 50%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 100%
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