Serotype and strain specific molecular differentiation of Marek's disease virus

Layton, R.D., and Burgess, G.W. (2002) Serotype and strain specific molecular differentiation of Marek's disease virus. In: Proceedings of the Australian Poultry Science Symposium 2002 (14) p. 198. From: 14th Annual Australian Poultry Science Symposium, 11-13 February 2002, Sydney, NSW, Australia.

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[Extract] Marek's disease (MD) is a lymphoproliferative disease of chickens caused by an avian herpesvirus, Marek's disease virus (MDV). There are three recognised serotypes (1, 2 and 3) of which only serotype 1 is pathogenic (Churchill & Biggs, 1967). Vaccination using all 'three serotypes is currently used as the mainstay for inducing protection against MD. The current method of detecting MDV post vaccination uses cell culture with immunofluorescence. PCRs have been designed using cumbersome phenol/chloroform protocols to extract DNA from feather tips and whole blood (Handberg et al., 2001). There is no current method that can separate different strains of type 1 MDV. The present study uses blood collected on filter paper and PCR to differentiate between serotypes and strains of type 1 following vaccination.

Item ID: 14681
Item Type: Conference Item (Scholarly Work)
ISSN: 1034-6260
Keywords: Maarek's disease; PCR; poultry; serotypes
Date Deposited: 20 Sep 2017 03:31
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0702 Animal Production > 070205 Animal Protection (Pests and Pathogens) @ 51%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070707 Veterinary Microbiology (excl Virology) @ 49%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830309 Poultry @ 100%
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