Hitchins, Sam, Cieslar, Julie M., and Dobson, Geoffrey P. (2001) 31P NMR quantitation of phosphorus metabolites in rat heart and skeletal muscle in vivo. American Journal of Physiology-Heart and Circulatory Physiology, 281 (2). H882-H887.

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Abstract

The aim of this study was to examine two methods of 31P NMR quantitation of phosphocreatine (PCr), ATP, and Pi in rat heart and skeletal muscle in vivo. The first method employed an external standard of phenylphosphonic acid (PPA; 10 mM), and the second method used an enzymatic measurement of tissue ATP equated to the area under the βATP peak. With the use of the external standard, the concentrations of ATP, PCr, and Pi in the rat heart were 4.48 ± 0.33, 9.21 ± 0.65, and 2.25 ± 0.16 μmol/g wet wt, respectively. With the use of the internal ATP standard, measured on the same tissue, the contents (means ± SE) were 4.78 ± 0.19, 9.83 ± 0.18, and 2.51 ± 0.33 μmol/g wet wt, respectively (n = 7). In skeletal muscle, ATP, PCr, and Pi were 6.09 ± 0.19, 23.44 ± 0.88, and 1.81 ± 0.18 μmol/g wet wt using the PPA standard and 6.03 ± 0.19, 23.30 ± 1.30, and 1.82 ± 0.19 μmol/g wet wt using the internal ATP standard (n = 6). There was no significant difference for each metabolite as measured by the two methods of quantification in heart or skeletal muscle. The results validate the use of an external reference positioned symmetrically above the coil and imply that each has similar NMR sensitivities (similar signal amplitude per mole of 31P between PPA and tissue phosphorus compounds). We conclude that PCr, ATP, and Pi are nearly 100% visible in the normoxic heart and nonworking skeletal muscle given the errors of measurement.

Item ID:	13067
Item Type:	Article (Research - C1)
ISSN:	1522-1539
Keywords:	heart muscle; phosphorus; skeletal muscle
Funders:	National Heart Foundation
Date Deposited:	28 Feb 2013 01:38
FoR Codes:	11 MEDICAL AND HEALTH SCIENCES > 1101 Medical Biochemistry and Metabolomics > 110199 Medical Biochemistry and Metabolomics not elsewhere classified @ 50% 11 MEDICAL AND HEALTH SCIENCES > 1199 Other Medical and Health Sciences > 119999 Medical and Health Sciences not elsewhere classified @ 25% 11 MEDICAL AND HEALTH SCIENCES > 1116 Medical Physiology > 111602 Human Biophysics @ 25%
SEO Codes:	92 HEALTH > 9202 Health and Support Services > 920203 Diagnostic Methods @ 50% 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920103 Cardiovascular System and Diseases @ 25% 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920116 Skeletal System and Disorders (incl. Arthritis) @ 25%
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