Characterisation of anthracyclines from a cosmomycin D-producing species of Streptomyces by collisionally-activated dissociation and ion mobility mass spectrometry

Kelso, Celine, Rojas, Juan Diego, Furlan, Renata L.A., Padilla, Gabriel, and Beck, Jennifer L. (2009) Characterisation of anthracyclines from a cosmomycin D-producing species of Streptomyces by collisionally-activated dissociation and ion mobility mass spectrometry. European Journal of Mass Spectrometry, 15 (2). pp. 73-81.

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Abstract

Cultures of cosmomycin D-producing Streptomyces olindensis ICB20 that were propagated for many generations underwent mutations that resulted in production of a range of related anthracyclines by the bacteria. The anthracyclines that retained the two trisaccharide chains of the parent compound were separated by HPLC. Exact mass determination of these compounds revealed that they differed from cosmomycin D (CosD) in that they contained one to three fewer oxygen atoms (loss of hydroxyl groups). Some of the anthracyclines that were separated by HPLC had the same mass. The location from which the hydroxyl groups had been lost relative to CosD (on the aglycone and/or on the sugar residues) was probed by collisionally- activated dissociation using an electrospray ionisation linear quadrupole ion trap mass spectrometer. The presence of anthracyclines with the same mass, but different structure, was confirmed using an electrospray ionisation travelling wave ion mobility mass spectrometer.

Item ID: 11452
Item Type: Article (Research - C1)
ISSN: 1751-6838
Keywords: anthracycline; linear quadrupole ion trap; MSn; travelling wave; ion mobility; electrospray ionisation mass spectrometry; collisionally-activated dissociation
Date Deposited: 04 Jun 2010 05:29
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060501 Bacteriology @ 50%
03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030401 Biologically Active Molecules @ 50%
SEO Codes: 86 MANUFACTURING > 8609 Veterinary Pharmaceutical Products > 860903 Veterinary Pharmaceutical Treatments (e.g. Antibiotics) @ 100%
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