Contractile effects of adenosine, coronary flow and perfusion pressure in murine myocardium

Willems, Laura, and Headrick, John P. (2007) Contractile effects of adenosine, coronary flow and perfusion pressure in murine myocardium. Pflügers Archiv European Journal of Physiology, 453 (4). pp. 433-441.

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There is mixed evidence adenosine receptors (ARs) may enhance myocardial contractility, although this remains contentious. We assessed inotropic actions of adenosine (50 μM) and selective AR activation with 100 nM N 6-cyclohexyladenosine (CHA; A1AR agonist), 25 nM 2-[p-(2-carboxyethyl) phenethylamino]-5′-N-ethylcarboxamidoadenosine (CGS-21680; A2AAR agonist) and 100 nM 2-chloro-N 6-(3-iodobenzyl)-adenosine-5′-N-methyluronamide (Cl-IB-MECA; A3AR agonist) in mouse hearts perfused at constant pressure, constant flow, or conditions of stable flow and pressure (following maximal nitroprusside-mediated dilatation at constant flow). Adenosine and CGS-21680 significantly (although modestly) increased force in constant-pressure perfused hearts (≤10 mmHg elevations in systolic pressure), effects paralleled by coronary vasodilatation (≤10 ml min−1 g−1 elevations in flow). Neither CHA nor Cl-IB-MECA altered force or flow. With constant-flow perfusion, adenosine and CGS-21680 reduced systolic pressure in parallel with perfusion pressure. When changes in coronary flow and pressure were prevented, CGS-21680 failed to alter contractility. However, adenosine still enhanced systolic pressure up to 10 mmHg. Relations between flow, perfusion pressure and ventricular force evidence substantial Gregg effects in murine myocardium: systolic force increases transiently by approximately 1 mmHg ml−1 min−1 g−1 rise in flow during the first minutes of hyperaemia and in a sustained manner (by ∼1 mmHg mmHg−1) during altered perfusion pressure. These effects contribute to inotropism with AR agonism when flow/pressure is uncontrolled. In summary, we find no evidence of direct A1 or A3AR-mediated inotropic responses in intact myocardium. Inotropic actions of A2AAR agonism appear entirely Gregg-related. Nonetheless, the endogenous agonist adenosine exerts a modest inotropic action independently of flow and perfusion pressure. The basis of this response remains to be identified.

Item ID: 10033
Item Type: Article (Research - C1)
ISSN: 1432-2013
Keywords: adenosine; adenosine receptor; coronary flow; myocardial contractility; gregg phenomenon; mouse heart
Date Deposited: 20 Apr 2010 02:51
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1199 Other Medical and Health Sciences > 119999 Medical and Health Sciences not elsewhere classified @ 50%
06 BIOLOGICAL SCIENCES > 0606 Physiology > 060699 Physiology not elsewhere classified @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920103 Cardiovascular System and Diseases @ 50%
97 EXPANDING KNOWLEDGE > 970102 Expanding Knowledge in the Physical Sciences @ 50%
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