Double-stranded oligonucleotides and uses therefor
Dixon, Nicholas, Schaeffer, Patrick, and Mulcair, Mark Australian National University (2006) Double-stranded oligonucleotides and uses therefor. WO 2006 081623 A1 .
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The present invention relates generally to the field of screening or diagnostic applications in which a target is required to be displayed for binding to another molecule, or interaction or reaction with another molecule. In particular, the present invention relates to the use of DNA/protein interactions to immobilize or present one or more biomolecules for screening purposes. The present invention more particularly relates to double-stranded oligonucleotides, wherein said oligonucleotide comprises a first strand and a second strand, wherein: (a) said first strand comprises the sequence: 5'-NC R ND G T T G T A A C N0 A-3' (SEQ ID NO: 1) or an analogue or derivative of said sequence; and (b) said second strand comprises the sequence: 5'-T ND G T T A C A A C ND T NC -3' (SEQ ID NO: 2) or an analogue or derivative of said sequence wherein R is a purine, NC and ND are each a DNA or RNA residue or analogue thereof, ND residues in said first strand and said second strand are sufficiently complementary to permit said ND residues to be annealed in the double-stranded oligonucleotide, and the sequence 5'-GTTGTAAC-3' (SEQ ID NO: 3) of said first strand is annealed to the complementary sequence 5' GTTACAAC-3' (SEQ ID NO: 4) of said second strand.
International Application Number: PCT/AU2006/000136; International Publication Number: WO 2006 081623 A1
|Date Deposited:||08 Aug 2012 23:15|
|FoR Codes:||10 TECHNOLOGY > 1003 Industrial Biotechnology > 100306 Industrial Molecular Engineering of Nucleic Acids and Proteins @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%|