Estradiol alters transcription factor gene expression in primate prefrontal cortex
Wang, J., Cheng, C.M., Zhou, J., Smith, A., Shannon Weickert, C., Perlman, W.R., Becker, K.G., Powell, D., and Bondy, C.A. (2004) Estradiol alters transcription factor gene expression in primate prefrontal cortex. Journal of Neuroscience Research, 76 (3). pp. 306-314.
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Estrogen protects neurons from a variety of experimental insults in vitro, and is thought to protect from acute and chronic neurodegenerative processes in vivo. Estrogen also enhances higher-level cognitive functions thatare centered in the dorsolateral prefrontal cortex (DLPFC) in human and non-human primates. To investigate genomic mechanisms involved in estrogenic effects on the primate brain in vivo, we compared transcription factor mRNA and protein expression in the DLPFC of ovariectomized rhesus monkeys treated with either vehicle or estradiol (E2). c-FOS, E2F1, and general transcription factor IIB (TFIIB) mRNA and protein expression were altered significantly by short-term E2 treatment, as shown by DNA array, in situ hybridization, and immunohistochemical and immunoblot evaluations. c-FOS expression was increased significantly whereas E2F1 and TFIIB levels were decreased in the DLPFC of E2-treated animals. These transcription factors were concentrated in cortical pyramids, as were estrogen receptors α and β. These data indicate that estrogen may have direct as well as indirect effects on neuronal gene expression in the primate prefrontal cortex.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||estrogen; brain; neuron; c-FOS; E2F1; TFIIB; rhesus monkey; memory; cognition|
|Date Deposited:||29 Mar 2010 03:42|
|FoR Codes:||11 MEDICAL AND HEALTH SCIENCES > 1109 Neurosciences > 110903 Central Nervous System @ 100%|
|SEO Codes:||92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920111 Nervous System and Disorders @ 100%|