Tracking antigen-specific lymphocytes in vivo
Adams, Claire L., Rush, Catherine M., Smith, Karen M., and Garside, Paul (2004) Tracking antigen-specific lymphocytes in vivo. Methods in Molecular Biology, 239. pp. 133-146.
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With the development of antigen-specific T-cell-receptor (TcR) and B-cell-receptor (BcR) transgenic (Tg) mice, it is now feasible to track single, antigen-specific lymphocyte populations throughout the course of an immune response. Adoptive transfer of the transgenic cell populations into naïve recipients with the same genetic background increases precursor frequency sufficiently to allow researchers to locate and functionally assess lymphocytes, in the physiological context of a normal animal, early after antigen exposure (1–4). Prior to the availability of such transgenic mice, it was extremely difficult to track lymphocytes in vivo during the early phases of an immune response before significant clonal expansion, because of the low precursor frequency of antigen-specific B- and T-cells. The following methods will illustrate some of the techniques now available for the tracking of antigen specific B- and T-cell populations in vivo.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||adoptive transfer; animals; antigens; cell division; cell movement; chemotaxis; Leukocyte; flow cytometry; fluorescent dyes Lymphocytes/cytology/*immunology/*physiology Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Transgenic Muramidase/immunology Ovalbumin/immunology Receptors, Antigen, T-Cell/metabolism Signal Transduction Th1 Cells/cytology/immunology/physiology Th2 Cells/cytology/immunology/physiology|
|Date Deposited:||22 Mar 2010 05:55|
|FoR Codes:||11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110704 Cellular Immunology @ 100%|
|SEO Codes:||92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 100%|