Development of DNA vaccines against Burkholderia pseudomallei

McAllister, Jane, Lazzaroni, Sharon, Barnes, Jodie, Ketheesan, Natkunam, and Proll, David (2009) Development of DNA vaccines against Burkholderia pseudomallei. Report. DSTO Defence Science and Technology Organisation, Fishermans Bend, VIC, Australia.

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Abstract

Burkholdena pseudomallei is the causative agent of melioidosis, a disease endemic in Southeast Asia and NorthemAustralia. The bacteria cause infection via subcutaneous or inhaled routes, resulting in either acute lethal sepsis or chronic and eventually fatal disease. Currently no licensed vaccine is available to provide protection against this pathogen. In this study, eleven DNA vaccines encoding the B. pseudomallei proteins were constructed and used to immunise mice and subsequent protective capability analysed following live challenge with B. pseudomallei.

Mice immunised with DNA vaccines encoding Hagl; Hag2 and heat shock protein demonstrated the greatest level of protection against bacterial infection, however, the levels of protection seen were still quite limited. There was no increase in survival seen following immunisation with the remaining eight vaccines. This study highlights the challenges in designing vaccines against B. pseudomallei and suggests that a successful DNA, vaccine may need to be used in conjunction with adjuvant such as CpG oligodeoxynuc1eotide, or combined in a prime-boost regime with recombinant protein in order to increase protective capabilities.

Item ID: 9255
Item Type: Report (Report)
Additional Information:

Report No: DSTO-TN-0872

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Date Deposited: 22 Dec 2010 01:09
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 70%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110704 Cellular Immunology @ 30%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 50%
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