An embryo-rescue protocol for Vigna interspecific hybrids

Palmer, J.L., Lawn, R.J., and Adkins, S.W. (2002) An embryo-rescue protocol for Vigna interspecific hybrids. Australian Journal of Botany, 50 (3). pp. 331-338.

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Abstract

A successful embryo-rescue and culture protocol was developed for use with several indigenous Vigna species and mungbean cultivars grown in Australia. Germination of Vigna immature embryos and their subsequent development into plants was influenced by the time at which the embryos were isolated and by which medium additives were placed in the embryo-rescue medium. A medium containing MS basal nutrients with sucrose (88 mM), casein hydrolysate (500 mg L-1) and agar (8 g L-1) but devoid of plant growth regulators was found to be the best for germination of immature embryos for all four Vigna species investigated. The protocol for successful germination of non-hybrid immature embryos was applied to the recovery of interspecific hybrids involving mungbean and five native Vigna species that had previously been found difficult to hybridise. Several putative hybrid plants were obtained including a confirmed interspecific cross between V. luteola (Jacq.) Benth and V. marina (Burm.) Merrill.

Item ID: 9181
Item Type: Article (Refereed Research - C1)
Keywords: genetic compatibility, hybrid breakdown, inter-specific hybridisation
ISSN: 1444-9862
Funders: Grains Research and Development Corporation
Projects and Grants: 7300.57478.1002
Date Deposited: 23 Nov 2010 23:54
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding) @ 100%
SEO Codes: 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8204 Summer Grains and Oilseeds > 820499 Summer Grains and Oilseeds not elsewhere classified @ 100%
Citation Count from Web of Science Web of Science 12
Downloads: Total: 2
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