Juxtaposition of C(2)M and the transverse filament protein C(3)G within the central region of Drosophila synaptonemal complex

Anderson, Lorinda K, Royer, Suzanne M, Page, Scott L, McKim, Kim S, Lai, Ann, Lilly, Mary A, and Hawley, R Scott (2005) Juxtaposition of C(2)M and the transverse filament protein C(3)G within the central region of Drosophila synaptonemal complex. Proceedings of the National Academy of Sciences of the United States of America, 102 (12). pp. 4482-4487.

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Abstract

The synaptonemal complex (SC) is intimately involved in the process of meiotic recombination in most organisms, but its exact role remains enigmatic. One reason for this uncertainty is that the overall structure of the SC is evolutionarily conserved, but many SC proteins are not. Two putative SC proteins have been identified in Drosophila: C(3)G and C(2)M. Mutations in either gene cause defects in SC structure and meiotic recombination. Although neither gene is well conserved at the amino acid level, the predicted secondary structure of C(3)G is similar to that of transversefilament proteins, and C(2)M is a distantly related member of the a-kleisin family that includes Rec8, a meiosis-specific cohesin protein. Here, we use immunogold labeling of SCs in Drosophila ovaries to localize C(3)G and C(2)M at the EM level. We show that both C(3)G and C(2)M are components of the SC, that the orientation of C(3)G within the SC is similar to other transverse-filament proteins, and that the N terminus of C(2)M is located in the central region adjacent to the lateral elements (LEs). Based on our data and the known phenotypes of C(2)M and C(3)G mutants, we propose a model of SC structure in which C(2)M links C(3)G to the LEs.

Item ID: 849
Item Type: Article (Refereed Research - C1)
Keywords: meiosis, synaptonemal complex, c(3)G, recombination, electron microscopy, drosophila melanogaster
Additional Information:

Copyright © 2005 by the National Academy of Sciences

ISSN: 1091-6490
Date Deposited: 23 Oct 2006
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060402 Cell and Nuclear Division @ 0%
06 BIOLOGICAL SCIENCES > 0699 Other Biological Sciences @ 0%
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