Isolation and characterization of 14 dinucleotide microsatellite loci in the endangered herb Saruma henryi Oliv. (Aristolochiaceae)

Dong, Shan-Shan, Huang, Xuan, Qian, Zeng-Qiang, Zhou, Tian-Hua, Li, Shan, and Zhao, Gui-Fang (2009) Isolation and characterization of 14 dinucleotide microsatellite loci in the endangered herb Saruma henryi Oliv. (Aristolochiaceae). Conservation Genetics, 1 (1). pp. 221-224.

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Abstract

Saruma henryi Oliv. (Aristolochiaceae) is an endangered perennial herb endemic to China, and has received considerable attention for its high phylogenetic, ecological and medicinal significances. To investigate its genetic diversity and structure, 14 polymorphic microsatellite loci were isolated and characterized from a dinucleotide-enriched genomic library of S. henryi. The number of alleles per locus varied between 5 and 8, with the average of 7.07 per locus. The expected and observed heterozygosities ranged from 0.6281 to 0.8532 and from 0.4762 to 0.8000, respectively. In addition, their cross-taxa transferability was also evaluated in two cofamilial species Aristolochia contorta Bge. and Asarum himalaicum Hook. f. et Thoms. ex Klotzsch. These informative microsatellite loci provide a powerful tool for genetic analyses of S. henryi and related taxa.

Item ID: 7863
Item Type: Article (Refereed Research - C1)
Keywords: Aristolochiaceae, codominant markers, edemic species, microsatellites, Saruma henryi Oliv., tropical biology
ISSN: 1572-9737
Date Deposited: 18 Dec 2009 00:45
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 60%
06 BIOLOGICAL SCIENCES > 0608 Zoology > 060808 Invertebrate Biology @ 40%
SEO Codes: 96 ENVIRONMENT > 9604 Control of Pests, Diseases and Exotic Species > 960404 Control of Animal Pests, Diseases and Exotic Species in Forest and Woodlands Environments @ 50%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
Citation Count from Web of Science Web of Science 2
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