A hypothetical model for VHML phage conversion of Vibrio harveyi
Oakey, Jane, Cullen, Bradford, and Owens, Leigh (2005) A hypothetical model for VHML phage conversion of Vibrio harveyi. In: Proceedings of the Fifth Symposium on Diseases in Asian Aquaculture, pp. 457-464. From: Diseases in Asian Aquaculture V, 24-28 November 2005, Gold Coast, QLD, Australia.
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The bacteriophage VHML (Vibrio harveyi myoviruslike) originated from a toxin producing strain of Vibrio harveyi. It has been demonstrated previously that the presence of the VHML prophage can induce virulence to V. harveyi in the laboratory. Here, a hypothetical model for phage virulence conversion of V. harveyi is presented. Through the nucleotide sequence determination of the entire VHML genome (43,193 bp), we have identified putative phage structural genes consistent with the physical characteristics of the virions as observed by TEM. We have also identified putative genes consistent with integration of the genome, supporting the theory that VHML integrates the host V. harveyi genome as a prophage. In addition, we have identified a potential toxin gene on the VHML genome. This gene includes DNA sequence similar to the reported active site of the ADP-ribosylating group of toxins. These ADP-RT's indude toxins from other bacteria reported previousiy to be a result of bacteriophage conversion. This presentation will illustrate how the phage genes could cause infection of VIbrio harveyi host cells, integration of the phage genome into the hosts' chromosome and subsequent production of the putative toxin, thereby conferring virulence to Vibrio harveyi.
|Item Type:||Conference Item (Refereed Research Paper - E1)|
|Date Deposited:||11 Jan 2010 23:41|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 100%|
|SEO Codes:||83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830199 Fisheries - Aquaculture not elsewhere classified @ 100%|