A rapid and repeatable method for venom extraction from Cubozoan nematocysts

Carrette, T., and Seymour, J. (2004) A rapid and repeatable method for venom extraction from Cubozoan nematocysts. Toxicon, 44 (2). pp. 135-139.

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Abstract

Various comparative studies into the biological activity and relative toxicity of cubozoan venoms have been investigated, in particular the venom from the potentially lethal cubozoan Chironex fleckeri. Efficient and reliable extraction of venom from nematocysts is essential before any research into venom toxicity can be conducted and previous cited methods of extraction have varied greatly, each with their own associated problems. A new standardised technique for the recovery of venom from nematocysts of cubozoans is investigated to decrease the variation displayed between authors due to differing extraction techniques. The use of a mini bead mill beater, as investigated in this trial, allows for the rapid extraction of venom from nematocysts and is devoid of the previously isolated problems experienced with other methods of venom isolation, such as excessive heat build up.

Item ID: 7192
Item Type: Article (Refereed Research - C1)
Keywords: Chironex fleckeri; Nematocyst; venom extraction
ISSN: 1879-3150
Date Deposited: 23 Mar 2010 03:51
FoR Codes: 06 BIOLOGICAL SCIENCES > 0608 Zoology > 060808 Invertebrate Biology @ 100%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960806 Forest and Woodlands Flora, Fauna and Biodiversity @ 100%
Citation Count from Web of Science Web of Science 40
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