DNA-binding characteristics of cnidarian Pax-C and Pax-B proteins in vivo and in vitro: no simple relationship with the Pax-6 and Pax-2/5/8 classes

Plaza, Serge, de Jong, Danielle M., Gehring, Walter J., and Miller, David J. (2003) DNA-binding characteristics of cnidarian Pax-C and Pax-B proteins in vivo and in vitro: no simple relationship with the Pax-6 and Pax-2/5/8 classes. Journal of Experimental Zoology Part B: molecular and developmental evolution, 299B (1). pp. 26-35.

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Abstract

Cnidarians are the simplest animals in which distinct eyes are present. We have previously suggested that cnidarian Pax-Cam might represent a precursor of the Pax-6 class. Here we show that when expressed in Drosophila imaginal discs, Pax-Cam chimeric proteins containing the C-terminal region of EY were capable of eye induction and driving expression of a reporter gene under the control of a known EY target (the sine oculis gene). Whilst these results are consistent with a Pax-6-like function for Pax-Cam, in band shift experiments we were unable to distinguish the DNA-binding behaviour of the Pax-Cam Paired domain from that of a second Acropora Pax protein, Pax-Bam. The ability of a Pax-Bam/EY chimera to also induce eye formation in leg imaginal discs, together with the in vitro data, cast doubt on previously assumed direct relationships between cnidarian Pax genes and the Pax-6 and Pax-2/5/8 classes of bilateral animals.

Item ID: 6581
Item Type: Article (Refereed Research - C1)
Keywords: Acropora millepora; Cnidaria; development; evolution; pax genes
ISSN: 1552-5015
Date Deposited: 09 Feb 2010 04:42
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060499 Genetics not elsewhere classified @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE @ 100%
Citation Count from Web of Science Web of Science 10
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