DNA-binding characteristics of cnidarian Pax-C and Pax-B proteins in vivo and in vitro: no simple relationship with the Pax-6 and Pax-2/5/8 classes
Plaza, Serge, de Jong, Danielle M., Gehring, Walter J., and Miller, David J. (2003) DNA-binding characteristics of cnidarian Pax-C and Pax-B proteins in vivo and in vitro: no simple relationship with the Pax-6 and Pax-2/5/8 classes. Journal of Experimental Zoology Part B: molecular and developmental evolution, 299B (1). pp. 26-35.
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Cnidarians are the simplest animals in which distinct eyes are present. We have previously suggested that cnidarian Pax-Cam might represent a precursor of the Pax-6 class. Here we show that when expressed in Drosophila imaginal discs, Pax-Cam chimeric proteins containing the C-terminal region of EY were capable of eye induction and driving expression of a reporter gene under the control of a known EY target (the sine oculis gene). Whilst these results are consistent with a Pax-6-like function for Pax-Cam, in band shift experiments we were unable to distinguish the DNA-binding behaviour of the Pax-Cam Paired domain from that of a second Acropora Pax protein, Pax-Bam. The ability of a Pax-Bam/EY chimera to also induce eye formation in leg imaginal discs, together with the in vitro data, cast doubt on previously assumed direct relationships between cnidarian Pax genes and the Pax-6 and Pax-2/5/8 classes of bilateral animals.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||Acropora millepora; Cnidaria; development; evolution; pax genes|
|Date Deposited:||09 Feb 2010 04:42|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0604 Genetics > 060499 Genetics not elsewhere classified @ 100%|
|SEO Codes:||97 EXPANDING KNOWLEDGE @ 100%|
|Citation Count from Web of Science||