6-Hydroxymelatonin protects against cyanide induced oxidative stress in rat brain homogenates

Maharaj, Deepa S., Walker, Roderick B., Glass, Beverley D., and Daya, Santy (2003) 6-Hydroxymelatonin protects against cyanide induced oxidative stress in rat brain homogenates. Journal of Chemical Neuroanatomy, 26 (2). pp. 103-107.

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Abstract

Both 6-hydroxymelatonin and N-acetyl-N-formyl-5-methoxykynurenamine are photodegradants and enzymatic metabolites of melatonin and are known to retain equipotent activity against potassium cyanide-induced superoxide generation compared to melatonin. It is not clear whether one or both of these metabolites is responsible for this effect. The present study therefore investigates the possible manner in which 6-hydroxymelatonin protects against oxidative stress induced by cyanide in rat brain homogenates. We examined the ability of 6-hydroxymelatonin to scavenge KCN-induced superoxide anion generation as well as lipid peroxidation. In addition, we also examined the effect of this indole on lactate dehydrogenase activity (LDH) as well as mitochondrial electron transport using dichlorophenol–indophenol as an electron acceptor. The results of this study show that 6-hydroxymelatonin significantly reduces KCN-induced superoxide anion generation, which is accompanied by a commensurate reduction in lipid peroxidation. Partial reversal of the KCN-induced reduction in mitochondrial electron transport is accompanied by a similar reversal of mitochondrial LDH activity blunted by KCN. It can thus be proposed that 6-hydroxymelatonin is potentially neuroprotective against KCN-induced neurotoxicity.

Item ID: 6539
Item Type: Article (Refereed Research - C1)
Keywords: 6-Hydroxymelatonin; electron transport chain; free radicals; lipid peroxidation; mitochondria; neurotoxicity; superoxide anion
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ISSN: 1873-6300
Date Deposited: 02 Mar 2010 01:14
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1115 Pharmacology and Pharmaceutical Sciences > 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified @ 100%
SEO Codes: 86 MANUFACTURING > 8608 Human Pharmaceutical Products > 860899 Human Pharmaceutical Products not elsewhere classified @ 100%
Citation Count from Web of Science Web of Science 14
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