Multiple near-identical genotypes of Schistosoma japonicum can occur in snails and have implications for population-genetic analyses

Yin, Mingbo, Hu, Wei, Mo, Xiaojin, Wang, Shengyue, Brindley, Paul J., McManus, Donald P., Davis, George M., Feng, Zheng, and Blair, David (2008) Multiple near-identical genotypes of Schistosoma japonicum can occur in snails and have implications for population-genetic analyses. International Journal for Parasitology, 38 (14). pp. 1681-1691.

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Abstract

We genotyped (using 16 or 17 microsatellite loci) numerous adult Schistosoma japonicum raised in rabbits exposed to pooled cercariae from small numbers of naturally infected snails from several localities in China. As expected, duplicate multi-locus genotypes (MLGs) were found among these worms. Additionally, many more MLGs, often near-identical, were found than snails used as sources of cercariae. Explanations for these results include (i) genotyping errors, (ii) development within each infected snail of multiple sibling miracidia and (iii) somatic mutation producing genetically varied cercariae from a single miracidium. To control for genotyping errors we re-analysed samples from many individual worms, including repeating the initial PCR. Explanations invoking the development of multiple sibling miracidia within a single snail are not likely to be correct because almost all duplicate MLGs fell within same-sex clusters in a principal coordinates analysis. We would expect both sexes to be represented in a multi-miracidium infection. In addition, we exposed several snails to infection by a single miracidium. One such snail, via an experimentally infected mouse, yielded 48 adult worms. The presence of at least nine near-identical MLGs among these worms was confirmed by re-genotyping. We regard somatic mutation as the most likely explanation for our results. The implications of multiple MLGs for population-genetic studies in S. japonicum are discussed.

Item ID: 6424
Item Type: Article (Refereed Research - C1)
Keywords: tropical biology; China; clonal replication; genotyping; microsatellites; multi-locus genotype; Oncomelania; Schistosoma japonicum; somatic mutation
ISSN: 1879-0135
Date Deposited: 26 Jan 2010 23:27
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 60%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060409 Molecular Evolution @ 40%
SEO Codes: 92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920404 Disease Distribution and Transmission (incl. Surveillance and Response) @ 100%
Citation Count from Web of Science Web of Science 17
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