Evidence for non-ribosomal peptide synthetase production of cereulide (the emetic toxin) in Bacillus cereus
Horwood, Paul F., Burgess, Graham W., and Oakey, H. Jane (2004) Evidence for non-ribosomal peptide synthetase production of cereulide (the emetic toxin) in Bacillus cereus. FEMS Immunology & Medical Microbiology, 236 (2). pp. 319-324.
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Little is known about the process whereby the emetic toxin (or cereulide) of Bacillus cereus is produced. Two cereulide-producing strains of B. cereus were cloned and sequenced following polymerase chain reaction (PCR) amplification with primers that were specific for conserved regions of non-ribosomal peptide synthetase (NRPS) genes. The cloned regions of the B. cereus strains were highly homologous to conserved regions of other peptide synthetase nucleotide sequences. Primers were designed for two variable regions of the NRPS gene sequence to ensure specificity for the emetic strains. A total of 86 B. cereus strains of known emetic or non-emetic activity were screened using these primers. All of the emetic strains (n=30) displayed a 188 bp band following amplification and gel electrophoresis. We have developed an improved method of identifying emetic strains of B. cereus and provided evidence that cereulide is produced by peptide synthetases.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||Bacillus cereus; Cereulide; emetic toxin; peptide synthetase; polymerase chain reaction|
|Date Deposited:||30 Mar 2010 05:08|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060599 Microbiology not elsewhere classified @ 100%|
|SEO Codes:||92 HEALTH > 9299 Other Health > 929999 Health not elsewhere classified @ 100%|
|Citation Count from Web of Science||