Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents

Druar, Chris, Yu, Fei, Barnes, Jodie L., Okinaka, Richard T., Chantratita, Narisara, Beg, Steve, Stratilo, Chad W., Olive, Andrew J., Soltes, Glenn, Russell, Michelle L., Limmathurotsakul, Direk, Norton, Robert E., Ni, Sally X., Picking, William. D., Jackson, Paul J., Stewart, Don I.H., Tsvetnitsky, Vadim, Picking, Wendy L., Cherwonogrodzky, John. W., Ketheesan, Natkunam, Peacock, Sharon J., and Wiersma, Erik J. (2008) Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents. FEMS Immunology & Medical Microbiology, 52 (1). pp. 78-87.

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Abstract

Burkholderia pseudomallei is a biothreat agent and an important natural pathogen, causing melioidosis in humans and animals. A type III secretion system (TTSS-3) has been shown to be critical for virulence. Because TTSS components from other pathogens have been used successfully as diagnostic agents and as experimental vaccines, it was investigated whether this was the case for BipB, BipC and BipD, components of B. pseudomallei's TTSS-3. The sequences of BipB, BipC and BipD were found to be highly conserved among B. pseudomallei and B. mallei isolates. A collection of monoclonal antibodies (mAbs) specific for each Bip protein was obtained. Most recognized both native and denatured Bip protein. Burkholderia pseudomallei or B. mallei did not express detectable BipB or BipD under the growth conditions used. However, anti-BipD mAbs did recognize the TTSS needle structures of a Shigella strain engineered to express BipD. The authors did not find that BipB, BipC or BipD are protective antigens because vaccination of mice with any single protein did not result in protection against experimental melioidosis. Enzyme-linked immunosorbent assay (ELISA) studies showed that human melioidosis patients had antibodies to BipB and BipD. However, these ELISAs had low diagnostic accuracy in endemic regions, possibly due to previous patient exposure to B. pseudomallei.

Item ID: 6331
Item Type: Article (Refereed Research - C1)
Keywords: Burkholderia pseudomallei; monoclonal antibody; type III secreation system; vaccine; diagnostic detection
ISSN: 1574-695X
Date Deposited: 18 Feb 2010 00:14
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110309 Infectious Diseases @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110801 Medical Bacteriology @ 25%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110704 Cellular Immunology @ 25%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
Citation Count from Web of Science Web of Science 27
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