Diversity and mode of transmission of ammonia-oxidizing archaea in marine sponges

Steger, Doris, Ettinger-Epstein, Piers, Whalan, Stephen, Hentschel, Ute, de Nys, Rocky, Wagner, Michael, and Taylor, Michael W. (2008) Diversity and mode of transmission of ammonia-oxidizing archaea in marine sponges. Environmental Microbiology, 10 (4). pp. 1087-1094.

[img] PDF (Published Version)
Restricted to Repository staff only

View at Publisher Website: http://dx.doi.org/10.1111/j.1462-2920.20...

Abstract

The model marine crenarchaeote 'Cenarchaeum symbiosum' is until now the only ammonia-oxidizing archaeon known from a marine sponge. Here, phylogenetic analyses based on the 16S rRNA and ammonia monooxygenase subunit A (amoA) genes revealed the presence of putative ammonia-oxidizing archaea (AOA) in a diverse range of sponges from the western Pacific, Caribbean and Mediterranean. amoA diversity was limited even between different oceans, with many of the obtained sequences (75.9%; n total = 83) forming a monophyletic, apparently sponge- (and coral-) specific lineage, analogous to those previously inferred from comparative 16S rRNA gene studies of sponge-associated microbes. The presence of AOA in sponge larvae, as detected by 16S rRNA and amoA PCR assays as well as by fluorescence in situ hybridization, suggests they are vertically transmitted and thus might be of importance for ammonia detoxification within the sponge.

Item ID: 6284
Item Type: Article (Refereed Research - C1)
Keywords: chondrilla-australiensis demospongiae; 16S rRNA; vertical transmission; OIRS; rhopaloeides-odorabile; cenarchaeum-symbiosum; phylogenetic analysis; black-sea; ocean; nitrification; crenarchaeota
ISSN: 1462-2920
Date Deposited: 20 Jan 2010 05:44
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060504 Microbial Ecology @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
Citation Count from Web of Science Web of Science 53
Downloads: Total: 1
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page