Rapid identification of all Aedes albopictus, Aedes scutellaris and Aedes Aegypti life stages using real-time polymerase chain reaction assays

Hill, Lydia A., Davis, Joseph B., Hapgood, George, Whelan, Peter I., Smith, Greg A., Ritchie, Scott A., Cooper, R.D., and Van der Hurk, Andrew F. (2008) Rapid identification of all Aedes albopictus, Aedes scutellaris and Aedes Aegypti life stages using real-time polymerase chain reaction assays. American Journal of Tropical Medicine and Hygiene, 79 (6). pp. 866-875.

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Abstract

In 2005, a widespread infestation of Aedes albopictus was discovered in the Torres Strait, the region between northern Australia and New Guinea. To contain this species, an eradication program was implemented in 2006. However, the progress of this program is impeded by the difficulty of morphologically separating Ae. albopictus larvae from the endemic species Aedes scutellaris. In this study, three real-time TaqMan polymerase chain reaction assays that target the ribosomal internal transcribed spacer 1 region were developed to rapidly identify Aedes aegypti, Ae. albopictus, and Ae. scutellaris from northern Australia. Individual eggs, larvae, pupae, and adults, as well as the species composition of mixed pools were accurately identified. The assay method was validated using 703 field-collected specimens from the Torres Strait.

Item ID: 5724
Item Type: Article (Refereed Research - C1)
Keywords: mosquito transmission; dengue; molecular identification
ISSN: 1476-1645
Date Deposited: 22 Dec 2009 03:18
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1117 Public Health and Health Services > 111701 Aboriginal and Torres Strait Islander Health @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1117 Public Health and Health Services > 111799 Public Health and Health Services not elsewhere classified @ 50%
SEO Codes: 92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920404 Disease Distribution and Transmission (incl. Surveillance and Response) @ 50%
92 HEALTH > 9203 Indigenous Health > 920399 Indigenous Health not elsewhere classified @ 50%
Citation Count from Scopus Scopus 9
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