Genetic diversity and differentiation in Chinese sour cherry Prunus pseudocerasus Lindl., and its implications for conservation

Li, Miao-Miao, Cai, Yu-Liang, Qian, Zeng-Qiang, and Zhao, Gui-Fang (2009) Genetic diversity and differentiation in Chinese sour cherry Prunus pseudocerasus Lindl., and its implications for conservation. Genetic Resources and Crop Evolution, 56 (4). pp. 455-464.

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Abstract

In this study, the genetic diversity and differentiation of 10 natural Prunus pseudocerasus Lindl. populations were investigated using inter-simple sequence repeat (ISSR) markers. Totally, 18 selected primers generated 150 loci, with an average of 8.33 bands per primer. The results showed that the percentage of polymorphic bands (PPB) was pretty low at the population level (PPB = 1.13–32%), but relatively high at the species level (PPB = 84%). Besides, a high level of genetic differentiation among populations was detected based on the gene differentiation coefficient (G ST = 0.7118) and the hierarchical analysis of molecular variance (AMOVA) (Φ ST = 64.53%, P < 0.001), in line with the low inter-population gene flow (N m = 0.2025). Moreover, Mantel test revealed a significant correlation between genetic and geographic distances among the populations (r = 0.5272, P < 0.005). The high level of intraspecific genetic diversity was probably related with its life history traits, while its small population size and the resultant high levels of genetic drift and inbreeding might explain the low genetic diversity within populations. The relatively high inter-population genetic differentiation was largely attributed to its small population size, habitat fragmentation, the mode of pollen and seed dispersal, and geographic isolation. Based on the present study, conservation strategies were proposed to preserve this valuable natural germplasm resource.

Item ID: 4859
Item Type: Article (Refereed Research - C1)
Keywords: cherry; genetic differentiation; genetic diversity; inter-simple sequence repeats; (ISSRs); Prunus pseudocerasus; Lindl; tropical biology
ISSN: 1573-5109
Date Deposited: 07 Aug 2009 04:45
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 70%
05 ENVIRONMENTAL SCIENCES > 0502 Environmental Science and Management > 050202 Conservation and Biodiversity @ 30%
SEO Codes: 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8202 Horticultural Crops > 820299 Horticultural Crops not elsewhere classified @ 50%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
Citation Count from Web of Science Web of Science 11
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