Meso-[{Ru(phen)2}2(mu-HAT)]4+: a high-affinity DNA hairpin probe {HAT = 1,4,5,8,9,12-hexaazatriphenylene; phen = 1,10-phenanthroline}

Smith, Jayden A., Morgan, Joy L., Turley, Adam G., Collins, J. Grant, and Keene, F. Richard (2006) Meso-[{Ru(phen)2}2(mu-HAT)]4+: a high-affinity DNA hairpin probe {HAT = 1,4,5,8,9,12-hexaazatriphenylene; phen = 1,10-phenanthroline}. Dalton Transactions , 26. pp. 3179-3187.

[img] PDF (Published Version) - Published Version
Restricted to Repository staff only

View at Publisher Website: http://dx.doi.org/10.1039/b601073c

Abstract

H NMR spectroscopy and fluorescent intercalator displacement (FID) assays have been used to investigate the DNA-binding abilities of two series of dinuclear polypyridyl ruthenium(II) complexes of the form [{Ru(L)2}2(µ-BL)]4+ {L = 2,2-bipyridine (bpy), 4,4-dimethyl-2,2-bipyridine (Me2bpy), 1,10-phenanthroline (phen), or 4,7-dimethyl-1,10-phenanthroline (Me2phen); BL = 2,2-bipyrimidine (bpm) or 1,4,5,8,9,12-hexaazatriphenylene (HAT)}. Preliminary FID surveys of these metal complexes against a variety of different oligonucleotides revealed that those complexes based upon the HAT bridging ligand induced greater fluorescence decreases in dye-bound DNA than did their bpm-bridged counterparts, suggesting a higher binding affinity by the HAT-bridged species. Furthermore, the greatest fluorescence decreases were typically observed in an oligonucleotide featuring a six-base hairpin loop. The apparent binding affinity of the metal complexes was also found to be a function of the stereochemistry and identity of the terminal ligands of the complex. The meso () stereoisomer generally induced greater fluorescence decreases than did either enantiomer ( or ), phen-based terminal ligands performed better than bpy-based terminal ligands, and those terminal ligands with methyl substituents demonstrated stronger apparent binding than did their non-methylated analogues. NMR experiments on meso-[{Ru(phen)2}2(µ-HAT)]4+ and meso-[{Ru(Me2phen)2}2(µ-HAT)]4+ demonstrated that both complexes bound with high affinity to the six-base hairpin oligonucleotide at the stem–loop interface and provided evidence to support stronger binding by the methylated species. meso-[{Ru(phen)2}2(µ-HAT)]4+ was found to bind poorly to duplex DNA and smaller four-base hairpin loops in FID and NMR experiments, whereas FID data suggest that the methylated analogue binds relatively strongly to most oligonucleotide sequences (the four- and six-base hairpins in particular). These results demonstrate that binding affinity can come at the expense of selectivity, with meso-[{Ru(phen)2}2(µ-HAT)]4+ proving to be an efficient compromise between the two as a high-affinity DNA hairpin probe.

Item ID: 4593
Item Type: Article (Refereed Research - C1)
Keywords: DNA binding; hairpin; dinuclear; ruthenium; stereochemistry
ISSN: 1477-9234
Date Deposited: 15 Jun 2009 05:28
SEO Codes: 97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 100%
Citation Count from Web of Science Web of Science 18
Downloads: Total: 4
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page