Sabourault, Cécile, Ganot, Philippe, Moya, Aurélie, and Furla, Paola (2012) Endosymbiosis drives transcriptomic adjustements and genomic adapatations in cnidarians. In: Proceedings of the 12th International Coral Reef Symposium. 7A. pp. 1-5. From: 12th International Coral Reef Symposium, 9-13 July 2012, Cairns, QLD, Australia.

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Abstract

To decipher inter-partner signaling within the cnidarian-dinoflagellate endosymbiosis, we developed genomic resources (cDNA library and microarrays) for the symbiotic sea anemone Anemonia viridis. Differential gene expression was quantified during thermal stress, with and without UV radiation, between symbiotic vs aposymbiotic specimens and gastroderm vs epidermis tissues. During stress time-course experiments, each stress showed a specific gene expression profile with very little overlap. We show that the major response to thermal stress is rapid (24 hours) but returns to the baseline levels after 2 days. UVR alone has little effect but potentiates thermal stress, as expression of a second set of genes becomes differentially expressed at day 5. Analysis of genes differentially expressed between symbiotic vs bleached and symbiotic vs stressed specimens defined a restricted subset of genes (Kern). Tissue specific expression mapping of Kern genes showed that many were specifically enhanced in the symbiotic cells (gastroderm). Altogether, these data define the Kern genes as major molecular components of the symbiotic interaction. Functional annotations highlighted several pathways including collagen fibrillogenesis, vesicular trafficking, lipid metabolism, calcium signaling, inorganic carbon transfer and cell death, that were modified by stress. Phylogenomic investigations of several Kern genes (calumenin, NPC2, SYM32, dermatopontin, and Rhbg) demonstrate that these issued from cnidarian specific duplication events, with the Kern member being preferentially expressed in the gastroderm and specifically responding to stress. Such host specific genes subfunctionalizations suggest both genomic and transcriptomic adaptations driven by the physiological constraints of endosymbiosis.

Item ID:	45245
Item Type:	Conference Item (Research - E1)
ISBN:	978-0-9808572-5-2
Keywords:	symbiosis, bleaching, functional genomics, genomic adaptation, thermal stress
Related URLs:	Organisation
Additional Information:	© Copyright belongs to the authors.
Date Deposited:	22 Aug 2016 23:54
FoR Codes:	06 BIOLOGICAL SCIENCES > 0604 Genetics > 060405 Gene Expression (incl Microarray and other genome-wide approaches) @ 100%
SEO Codes:	97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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