In vitro effect of vitellogenin on steroid production by ovarian follicles of greenback flounder Rhombosolea taprina

Sun, B., and Pankhurst, N.W. (2006) In vitro effect of vitellogenin on steroid production by ovarian follicles of greenback flounder Rhombosolea taprina. Comparative Biochemistry and Physiology - Part A: molecular and Integrative physiology, 144 (1). pp. 78-85.

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Abstract

Ovarian follicles from vitellogenic greenback flounder (Rhombosolea tapirina) were incubated in L15 medium alone, or containing human chorionic gonadotropin (hCG), dibutyryl cyclic AMP (dbcAMP) or the steroid precursors testosterone (T), 17-hydroxyprogesterone (17P) and androstenedione (A) in the presence of vitellogenin (Vtg) at 0.1–5.0 mg mL− 1. Medium concentrations of 17β−estradiol (E2) and T were measured by radioimmunoassay. HCG generally stimulated follicular E2 but not T production, whereas 17P, A and T stimulated production of E2, T, and E2 respectively. Treatment of follicles with dbcAMP inhibited follicular E2 production, but increased follicular T production at high doses. The effect of low concentrations of Vtg on follicular steroid production was variable; however, higher doses of Vtg significantly suppressed basal, hCG-, dbcAMP- and steroid precursor-stimulated follicular E2 and T production. The results of this study show that high concentrations of Vtg may suppress follicular steroid production by interfering in the steroidogenic pathway. This suggests that Vtg may regulate its own production by limiting the ovarian production of E2.

Item ID: 3912
Item Type: Article (Refereed Research - C1)
Keywords: 17B-estradiol; ovarian follicle; greenback flounder; reproduction; steroidogenesis; testosterone; vitellogenin
ISSN: 1531-4332
Date Deposited: 19 Nov 2009 01:15
FoR Codes: 10 TECHNOLOGY > 1099 Other Technology > 109999 Technology not elsewhere classified @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830199 Fisheries - Aquaculture not elsewhere classified @ 100%
Citation Count from Web of Science Web of Science 1
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