Development of a microsatellite DNA parentage marker suite for black tiger shrimp Penaeus monodon
Jerry, Dean R., Evans, Brad S., Kenway, Matt, and Wilson, Kate (2006) Development of a microsatellite DNA parentage marker suite for black tiger shrimp Penaeus monodon. Aquaculture, 255 (1-4). pp. 542-547.
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Twenty-three published Penaeus monodon microsatellites were screened for inclusion in a DNA parentage marker suite by examining their ease of amplification, reliability of allele calling and Mendelian inheritance in an Australian captive population. Seven highly polymorphic markers (mean He = 0.87 and PIC = 0.84) were identified from the original 23 as being suitable for inclusion in the parentage marker suite. These seven microsatellites were evaluated for their combined ability to assign parentage to progeny from 13 families where genealogy was known. All progeny were correctly assigned to their true parents highlighting the usefulness of this marker suite to retain pedigree information in this species. Further, simulations based on broodstock allele frequencies suggested that the marker suite would be robust enough to identify progeny from up to 20 communally stocked families.
This study highlights the importance of properly evaluating individual microsatellite markers before undertaking parentage analyses.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||DNA parentage; microsatelites; black tiger shrimp; pedigreeing; Penaeus monodon|
|Date Deposited:||25 Oct 2009 22:55|
|SEO Codes:||83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830199 Fisheries - Aquaculture not elsewhere classified @ 100%|
|Citation Count from Web of Science||