Production of polyclonal antibodies to Batrachochytrium dendrobatidis and their use in an immunoperoxidase test for chytridiomycosis in amphibians

Berger, Lee, Hyatt, Alex D., Olsen, Veronica, Hengstberger, Sandra G., Boyle, Donna, Marantelli, Gerry, Humphreys, Kaye, and Longcore, Joyce (2002) Production of polyclonal antibodies to Batrachochytrium dendrobatidis and their use in an immunoperoxidase test for chytridiomycosis in amphibians. Diseases of Aquatic Organisms, 48 (3). pp. 213-220.

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Abstract

Polyclonal antibodies were produced for diagnosing chytridiomycosis in amphibians. Two sheep and 4 rabbits were inoculated with homogenized whole culture of Batrachochytrium dendrobatidis in Freund¹s complete adjuvant or triple adjuvant. Antisera from all animals reacted strongly with all stages of B. dendrobatidis and stained the walls, cytoplasm, rhizoids and zoospores in an indirect immunoperoxidase test. Significant cross-reactivity occurred only with some fungi in the Chytridiomycota, and there are no members of this phylum besides B. dendrobatidis that infect frogs. The immunoperoxidase stain is a useful screening test when combined with recognition of the morphology and infection site of B. dendrobatidis.

Item ID: 373
Item Type: Article (Refereed Research - C1)
Keywords: chytridiomycosis, antibodies
Additional Information:

Copyright 2002 Inter-Research

ISSN: 1616-1580
Date Deposited: 26 Oct 2006
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES @ 0%
Citation Count from Web of Science Web of Science 34
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