Cloning and expression of maize-leaf pyruvate, Pi dikinase regulatory protein gene

Burnell, Jim N., and Chastain, Chris J. (2006) Cloning and expression of maize-leaf pyruvate, Pi dikinase regulatory protein gene. Biochemical and Biophysical Research Communications, 345 (2). pp. 675-680.

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Abstract

Pyruvate, orthophosphate dikinase (PPDK; E.C. 2.7.9.1) catalyzes the synthesis of the primary inorganic carbon acceptor, phosphoenolpyruvate in the C4 photosynthetic pathway and is reversibly regulated by light. PPDK regulatory protein (RP), a bifunctional serine/threonine kinase-phosphatase, catalyzes both the ADP-dependent inactivation and the Pi-dependent activation of PPDK. Attempts to clone the RP have to date proven unsuccessful. A bioinformatics approach was taken to identify the nucleotide and amino acid sequence of the protein. Based on previously established characteristics including molecular mass, known inter- and intracellular location, functionality, and low level of expression, available databases were interrogated to ultimately identify a single candidate gene. In this paper, we describe the nucleotide and deduced amino acid sequence of this gene and establish its identity as maize PPDK RP by in vitro analysis of its catalytic properties via the cloning and expression of the recombinant protein.

Item ID: 3640
Item Type: Article (Refereed Research - C1)
Keywords: dikinase; C4; phosphorylation; PPDK; PPDK regulatory protein; RP; C4 photosynthesis; protein phosphorylation; dephosphorylation; maize; Zea mays
ISSN: 1090-2104
Date Deposited: 17 Jun 2009 04:30
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060104 Cell Metabolism @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE @ 100%
Citation Count from Web of Science Web of Science 20
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