Effect of inoculum to substrate ratio on the hydrolysis and acidification of food waste in leach bed reactor

Xu, S.Y., Karthikeyan, O.P., Selvam, A., and Wong, J.W. (2012) Effect of inoculum to substrate ratio on the hydrolysis and acidification of food waste in leach bed reactor. Bioresource Technology, 126. pp. 425-430.

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Abstract

The aim of present study was to determine an appropriate ISR (inoculum to substrate ratio) to enhance the hydrolysis rate and reduce the solid retention time of food waste in hydrolytic-acidogenesis leach bed reactor (LBR). LBR 1–4 were inoculated with 0%, 5%, 20% and 80% (w/w basis) of anaerobically digested sludge, respectively, using artificial food waste as substrate. Experiments were conducted in batch mode at mesophilic condition (35 °C) for 17 days. Higher ISR resulted in 4.3-fold increase in protein hydrolysis; whereas, only a modest increase in the decomposition of carbohydrate. Two kinetic models for carbohydrate and protein degradation were proposed and evaluated. The differences among four ISRs in volatile solids removal efficiencies were marginal, i.e. 52.4%, 62.8%, 63.2% and 71.7% for LBR 1–4, respectively; indicating that higher ISR was insignificant in enhancing the overall hydrolysis rate in LBR. Therefore, a lower ISR of 20% was recommended in the hydrolytic-acidogenic process.

Item ID: 35922
Item Type: Article (Research - C1)
ISSN: 1873-2976
Keywords: acidogenesis; anaerobic digestion; inoculum to substrate ratio; kinetics study; proteolytic bacteria
Date Deposited: 27 Oct 2014 23:38
FoR Codes: 05 ENVIRONMENTAL SCIENCES > 0502 Environmental Science and Management > 050205 Environmental Management @ 40%
05 ENVIRONMENTAL SCIENCES > 0502 Environmental Science and Management > 050209 Natural Resource Management @ 30%
09 ENGINEERING > 0907 Environmental Engineering > 090703 Environmental Technologies @ 30%
SEO Codes: 85 ENERGY > 8505 Renewable Energy > 850501 Biofuel (Biomass) Energy @ 50%
90 COMMERCIAL SERVICES AND TOURISM > 9004 Water and Waste Services > 900401 Waste Management Services @ 50%
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