Translation of in vitro inhibition by marine natural products of the C4 acid cycle enzyme pyruvate Pi dikinase to in vivo C4 plant tissue death

Haines, Dianne S., Burnell, James N., Doyle, Jason R., Llewellyn, Lyndon E., Motti, Cherie A,, and Tapiolas, Dianne M. (2005) Translation of in vitro inhibition by marine natural products of the C4 acid cycle enzyme pyruvate Pi dikinase to in vivo C4 plant tissue death. Journal of Agricultural and Food Chemistry, 53 (10). pp. 3856-3862.

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Abstract

Marine organism derived extracts, previously identified as containing compounds that inhibited the C4 acid cycle enzyme pyruvate Pi dikinase (PPDK), were assessed for their ability to exhibit an effect on the C4 plants Digitaria ciliaris and Echinochloa crus-galli. Oxygen electrode studies revealed that over half of these extracts inhibited C4 acid driven photosynthesis in leaf slices. Seventeen extracts had a deleterious effect on C4 plants in vivo within 24 h, whereas 36 caused an observable phytotoxic response in one or both of the C4 plants used for in vivo testing. None of the extracts inhibited PPDK metabolism of pyruvate via a directly competitive mechanism, instead hindering the enzyme by either mixed or uncompetitive means. This screening strategy, using a suite of assays, led to the isolation and identification of the herbicidal marine natural product ilimaquinone.

Item ID: 3448
Item Type: Article (Refereed Research - C1)
Keywords: herbicide; PPDK; C4 plant; oxygen evolution
ISSN: 1520-5118
Date Deposited: 19 Jun 2009 00:33
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060107 Enzymes @ 100%
SEO Codes: 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8299 Other Plant Production and Plant Primary Products > 829999 Plant Production and Plant Primary Products not elsewhere classified @ 100%
Citation Count from Web of Science Web of Science 7
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