Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni

Pearson, M.S, McManus, D.P., Smyth, D.J., Jones, M.K., Sykes, A.M., and Loukas, A. (2007) Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni. Parasitology, 134 (14). pp. 2001-2008.

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Abstract

A partial cDNA sequence was obtained from the human blood fluke, Schistosoma mansoni using a signal sequence trap approach. The full-length cDNA was cloned and termed Sm-7TM. The corresponding open reading frame had 7 membrane spanning domains and shared identity with a small, novel group of seven transmembrane (7TM) receptors from vertebrates and invertebrates, including the human ee3 receptor - a heptahelical protein implicated in neuronal signalling. Phylogenetic analysis of this novel family showed that the Sm-7TM ORF formed a clade with exclusively invertebrate sequences. Based on topology modelling with ee3, Sm-7TM was predicted to possess an intracellular C-terminal tail, which was expressed as a soluble thioredoxin fusion protein (Sm-7TMC) in Escherichia coli and purified using metal ion-affinity chromatography. A polyclonal antiserum against this domain was used to detect Sm-7TM in detergent-soluble parasite extracts and to immunolocalize the receptor to the tegument of adult S. mansoni.

Item ID: 34143
Item Type: Article (Research - C1)
ISSN: 1469-8161
Keywords: Schistosoma mansoni, seven transmembrane receptor, tegument
Funders: National Health and Medical Research Council of Australia (NHMRC)
Date Deposited: 30 Jul 2014 04:36
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060599 Microbiology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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