Warren, Hilary S., Wu, Fan, Horn, Peggy L., Pyne, David B., West, Nicholas P., and Cripps, Allan W. (2013) Peripheral blood natural killer (NK) cell function in healthy adults assessed using the target-induced NK loss (TINKL) assay. Journal of Immunological Methods, 392 (1-2). pp. 68-70.

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Abstract

In this technical note we provide data useful for the clinical application of the target-induced Natural Killer (NK) loss (TINKL) assay. The TINKL assay is a sensitive flow cytometry-based assay for measuring NK cell function. Loss of NK cells from the lymphocyte gate occurs following culture with K562 (the prototypic target cell for natural killing) and antibody-coated target cells (for antibody-dependent killing). By analysis of multiple samples of PBMC from single donors we document the intra-experimental variability and the inter-experimental variability of the assay. The intra-experimental coefficient of variation (CV) was on average 11% for natural killing and 3% for antibody-dependent killing, compared to 14% and 9% respectively for the inter-experimental variation. Analysis of a 123 normal healthy adults shows large variability in the functional capacity of NK cells in the population both for natural killing (CV 33%) and antibody-dependent killing (CV 27%).

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