Effects of salinity, pH and temperature on the re-establishment of bioluminescence and copper or SDS toxicity in the marine dinoflagellate Pyrocystis lunula using bioluminescence as an endpoint

Craig, J.M., Klerks, Paul, Heimann, Kirsten R.M., and Waits, J.L. (2003) Effects of salinity, pH and temperature on the re-establishment of bioluminescence and copper or SDS toxicity in the marine dinoflagellate Pyrocystis lunula using bioluminescence as an endpoint. Environmental Pollution, 125 (2). pp. 267-275.

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Abstract

Pyrocystis lunula is a unicellular, marine, photoautotrophic, bioluminescent dinoflagellate. This organism is used in the Lumitox((R)) bioassay with inhibition of bioluminescence re-establishment as the endpoint. Experiments determined if acute changes in pH, salinity, or temperature had an effect on the organisms' ability to re-establish bioluminescence, or on the bioassay's potential to detect sodium dodecyl sulfate (SDS) and copper toxicity. The re-establishment of bioluminescence itself was not very sensitive to changes in pH within the pH 6-10 range, though reducing pH from 8 to levels below 6 decreased this capacity. Increasing the pH had little effect on Cu or SDS toxicity, but decreasing the pH below 7 virtually eliminated the toxicity of either compound in the bioassay. Lowering the salinity from 33 to 27parts per thousand or less resulted in a substantial decrease in re-establishment of bioluminescence, while increasing the salinity to 43 or 48 parts per thousand resulted in a small decline. Salinity had little influence on the bioassay's quantification of Cu toxicity, while the data showed a weak negative relationship between SDS toxicity and salinity. Re-establishment of bioluminescence showed a direct dependence on temperature, but only at 10 degreesC did temperature have an obvious effect on the toxicity of Cu. in this bioassay.

Item ID: 326
Item Type: Article (UNSPECIFIED)
Keywords: Pyrocystis lunula, Bioluminescence, Toxicity-testing, copper, SDS, Temperature, Salinity, pH, Hediste Nereis Diversicolor, Dependent toxicity, Gonyaulax-Polyedra, Aquatic organisms, Metal toxicity, Luciferase, Algae
Additional Information:

© 2003 Elsevier. This journal is available online - use hypertext links above.

ISSN: 1873-6424
Date Deposited: 13 Sep 2006
FoR Codes: 06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060701 Phycology (incl Marine Grasses) @ 0%
06 BIOLOGICAL SCIENCES > 0607 Plant Biology @ 0%
06 BIOLOGICAL SCIENCES > 0699 Other Biological Sciences @ 0%
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