Lessons from a beetle and an ant: coping with taxon-dependent differences in microsatellite development success
Arthofer, Wolfgang, Schlick-Steiner, Birgit C., Steiner, Florian M., Avtzis, Dimitrios N., Crozier, Ross H., and Stauffer, Christian (2007) Lessons from a beetle and an ant: coping with taxon-dependent differences in microsatellite development success. Journal of Molecular Evolution, 65 (3). pp. 304-307.
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Microsatellites are powerful markers often isolated de novo for species yet to be investigated. Enriched genomic libraries are usually used for isolation purposes. We critically evaluate the outcome of an enrichment-based protocol applied to two insect species (the ant Lasius austriacus and the beetle Pityogenes chalcographus) which yielded contrasting numbers of suitable loci. Our findings of differences in microsatellite isolation are consistent with the available data on differences in genomic characteristics across these taxa. In the beetle repeated isolation of identical motifs, difficulties in primer development, and multibanded products caused loss of most candidate clones. We identified critical steps during marker development.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||microsatellites; microsatellite isolation; enriched genomic library; molecular marker; informative locus; Coleoptera; Hymenoptera|
|Date Deposited:||22 Sep 2009 04:54|
|FoR Codes:||06 BIOLOGICAL SCIENCES > 0604 Genetics > 060409 Molecular Evolution @ 50%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 50%
|SEO Codes:||97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%|
|Citation Count from Web of Science||