Single nucleotide +1 frameshifts in an apparently functional mitochondrial cytochrome b gene in ants of the genus Polyrhachis

Beckenbach, Andrew T., Robson, Simon K.A., and Crozier, Ross H. (2005) Single nucleotide +1 frameshifts in an apparently functional mitochondrial cytochrome b gene in ants of the genus Polyrhachis. Journal of Molecular Evolution, 60 (2). pp. 141-152.

[img] PDF (Published Version)
Restricted to Repository staff only

View at Publisher Website: http://dx.doi.org/10.1007/s00239-004-017...

Abstract

Twelve of 30 species examined in the ant genus Polyrhachis carry single nucleotide insertions at one or two positions within the mitochondrial cytochrome b (cytb) gene. Two of the sites are present in more than one species. Nucleotide substitutions in taxa carrying insertions show the strong codon position bias expected of functional protein coding genes, with substitutions concentrated in the third positions of the original reading frame. This pattern of evolution of the sequences strongly suggests that they are functional cytb sequences. This result is not the first report of +1 frameshift insertions in animal mitochondrial genes. A similar site was discovered in vertebrates, where single nucleotide frameshift insertions in many birds and a turtle were reported by Mindell et al. (Mol Biol Evol 15:1568, 1998). They hypothesized that the genes are correctly decoded by a programmed frameshift during translation. The discovery of four additional sites gives us the opportunity to look for common features that may explain how programmed frameshifts can arise. The common feature appears to be the presence of two consecutive rare codons at the insertion site. We hypothesize that the second of these codons is not efficiently translated, causing a pause in the translation process. During the stall the weak wobble pairing of the tRNA bound in the peptidyl site of the ribosome, together with an exact Watson–Crick codon–anticodon pairing in the +1 position, allows translation to continue in the +1 reading frame. The result of these events is an adequate level of translation of a full-length and fully functional protein. A model is presented for decoding of these mitochondrial genes, consistent with known features of programmed translational frameshifting in the yeast TY1 and TY3 retrotransposons.

Item ID: 278
Item Type: Article (Refereed Research - C1)
Keywords: Frameshifts, mt-dna-sequence, cob, cytb, Molecular-evolution, Gene-expression translation, Formicine-ants, Weaver-ants, Polyrhachis, Programmed-translational-frameshifting, t-rna indels
Additional Information:

© 2005 Springer : The original publication is available at SpringerLink (use hypertext links above) http://www.springerlink.com

ISSN: 1432-1432
Date Deposited: 11 Sep 2006
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060407 Genome Structure and Regulation @ 100%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060409 Molecular Evolution @ 0%
06 BIOLOGICAL SCIENCES > 0608 Zoology > 060808 Invertebrate Biology @ 0%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
Citation Count from Web of Science Web of Science 22
Downloads: Total: 2
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page