A germline clone screen for meiotic mutants in Drosophila melanogaster

Page, Scott L., Nielsen, Rachel J., Teeter, Kathy, Lake, Cathleen M., Ong, SengKai, Wright, Kathleen R., Dean, Kristen L., Agne, Daniel, Gilliland, William D., and Hawley, R. Scott (2007) A germline clone screen for meiotic mutants in Drosophila melanogaster. Fly, 1 (3). pp. 172-181.

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Abstract

Using an FLP/FRT‑based method to create germline clones, we screened Drosophila chromosome arms 2L and 3R for new female meiotic mutants. The screen was designed to recover mutants with severe effects on meiotic exchange and/or segregation. This screen yielded 11 new mutants, including six alleles of previously known meiotic genes (c(2)M and ald/mps1). The remaining five mutants appear to define at least four new genes whose ablation results in severe meiotic defects. Three of the novel meiotic mutants were identified at the molecular level. Two of these, mcm5A7 and tremF9, define roles in meiotic recombination, while a third, conaA12, is important for synaptonemal complex assembly. Surprisingly, five of the nine mutants for which the lesion has been identified at the molecular level are not the result of mutations characteristic of EMS mutagenesis, but rather due to the insertion of the transposable element Doc. This study demonstrates the utility of germline clone‑based screens for the discovery of strong meiotic mutants, including mutations in essential genes, and the use of molecular genetic techniques to map the loci.

Item ID: 2657
Item Type: Article (Refereed Research - C1)
Keywords: meiosis, chromosome, genetic screen, recombination, germline, SNP
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Reproduced with permission from the journal Fly.

Page, Scott L., Nielsen, Rachel J., Teeter, Kathy, Lake, Cathleen M., Ong, SengKai, Wright, Kathleen R., Dean, Kristen L., Agne, Daniel, Gilliland, William D., and Hawley, R. Scott (2007) A germline clone screen for meiotic mutants in Drosophila melanogaster. Fly, 1 (3). pp. 172-181.

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ISSN: 1933-6942
Date Deposited: 29 Jul 2009 05:47
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060402 Cell and Nuclear Division @ 50%
06 BIOLOGICAL SCIENCES > 0604 Genetics > 060407 Genome Structure and Regulation @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920114 Reproductive System and Disorders @ 100%
Citation Count from Web of Science Web of Science 10
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