Deficiency of invariant NK T cells in Crohn's disease and ulcerative colitis
Grose, Randall H., Thompson, Fiona M., Baxter, Alan G., Pellicci, Daniel G., and Cummins, Adrian G. (2007) Deficiency of invariant NK T cells in Crohn's disease and ulcerative colitis. Digestive Diseases and Sciences, 52 (6). pp. 1415-1422.
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The aim of this study was to investigate whether immunoregulatory invariant NK T cells are deficient in Crohn's disease or ulcerative colitis. Blood was collected for flow cytometry from 106 Crohn's disease, 91 ulcerative colitis, and 155 control subjects. Invariant NK T cells were assessed by Vα24 and (α-galactosylceramide/CD1d tetramer markers. Intracellular cytokine was measured after in vitro anti-CD3 antibody stimulation. Vα24+ T cells were quantified in ileocolonic biopsies as mRNA by real-time PCR and by immunofluorescence. Circulating invariant NK T cells were 5.3% of the control levels in Crohn's (P < 0.001) and 7.9% of the control levels in ulcerative colitis (P < 0.001). Interleukin-4 production was impaired in Crohn's disease and ulcerative colitis. Intestinal Vα24 mRNA expression was 7% in Crohn's disease (P < 0.05) and 9% in ulcerative colitis (P < 0.05). Intestinal Vα24+ T cells were 23% in Crohn's disease but not reduced in ulcerative colitis. We conclude that invariant NK T cells are deficient in Crohn's disease and in ulcerative colitis.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||Crohn's disease; NKT cells; colitis|
|Date Deposited:||25 Sep 2009 01:57|
|FoR Codes:||11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110799 Immunology not elsewhere classified @ 100%|
|SEO Codes:||92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 100%|
|Citation Count from Web of Science||