A polymerase chain reaction-based diagnostic to identify larvae and eggs of container mosquito species from the Australian region
Beebe, Nigel W., Whelan, Peter I., van den Hurk, Andrew F., Ritchie, Scott A., Corcoran, Sarah, and Cooper, Robert D. (2007) A polymerase chain reaction-based diagnostic to identify larvae and eggs of container mosquito species from the Australian region. Journal of Medical Entomology, 44 (2). pp. 376-380.
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Dengue outbreaks occur regularly in parts of northern Queensland, Australia, and there is concern that these outbreaks may spread with the introduction and range expansion of the two main vectors Aedes aegypti (L.) and Aedes albopictus (Skuse). Problems encountered in separating larvae of endemic and exotic container mosquito species resulted in the development of a polymerase chain reaction diagnostic procedure that uses a restriction enzyme to cut the internal transcribed spacer region 1 of the ribosomal DNA to separate Ae. aegypti and Ae. albopictus from a number of common local container mosquito species which can be used at any stage of the life cycle, including eggs up to 8 wk of age. Identification was possible using desiccated or alcohol-preserved specimens with a response time of <24 h after receipt of the specimens.
|Item Type:||Article (Refereed Research - C1)|
|Keywords:||polymerase chain reaction diagnostic, container mosquito species, internal transcribed spacer region 1, Aedes mosquitoes|
|Date Deposited:||23 Jul 2009 05:37|
|FoR Codes:||11 MEDICAL AND HEALTH SCIENCES > 1117 Public Health and Health Services > 111799 Public Health and Health Services not elsewhere classified @ 100%|
|SEO Codes:||92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920405 Environmental Health @ 51%
92 HEALTH > 9299 Other Health > 929999 Health not elsewhere classified @ 49%
|Citation Count from Web of Science||