The in vitro vascular effects of two chirodropid (Chironex fleckeri and Chiropsella bronzie) venoms

Winter, Kelly L., Fernando, Ross, Ramasamy, Sharmaine, Seymour, Jamie E., Isbister, Geoffrey K., and Hodgson, Wayne C. (2007) The in vitro vascular effects of two chirodropid (Chironex fleckeri and Chiropsella bronzie) venoms. Toxicology Letters, 168 (1). pp. 13-20.

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Abstract

Clinical observations suggest a primary cardiotoxic role in fatal Chironex fleckeri stings. The limited research available indicates that Chiropsella bronzie venom acts in a similar manner although appears to be less potent. The aim of the present study was to elucidate the vascular effects of C. fleckeri and C. bronzie venoms using rat isolated aorta. Both venoms produced a sustained contraction of endothelium-denuded aorta which was not significantly affected by prazosin or box jellyfish antivenom. Felodipine significantly reduced the contractile response to C. fleckeri venom but not C. bronzie venom. Both venoms produced an initial relaxation (Phase 1), followed by a sustained contraction (Phase 2), in pre-contracted endothelium-intact aorta. Removal of the endothelium significantly inhibited both phases of the response. NOLA significantly inhibited Phase 1, but not Phase 2, of the response to both venoms. Atropine, HOE 140 or BQ 123 did not have any significant inhibitory effect on either phase. In conclusion, neither C. fleckeri nor C. bronzie venoms appear to contain components with activity at α1-adrenoceptors. Antivenom was ineffective in reversing the effects of the venom suggesting it is incapable of completely neutralising nematocyst-derived venom. Determining the mechanism of action of these venoms will allow for the development of better treatment strategies.

Item ID: 2522
Item Type: Article (Refereed Research - C1)
Keywords: box jellyfish; rat aorta; venom; vascular biology; Chironex fleckeri; Chiropsella bronzie
ISSN: 1879-3169
Date Deposited: 23 Jul 2009 01:13
FoR Codes: 06 BIOLOGICAL SCIENCES > 0606 Physiology > 060604 Comparative Physiology @ 100%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960899 Flora, Fauna and Biodiversity of Environments not elsewhere classified @ 100%
Citation Count from Web of Science Web of Science 19
Downloads: Total: 3
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