Acute toxicity of ammonia and its effects on the haemolymph osmolality, ammonia-N, pH and ionic composition of early juvenile mud crabs, Scylla serrata (Forskål)

Romano, Nicholas, and Zeng, Chaoshu (2007) Acute toxicity of ammonia and its effects on the haemolymph osmolality, ammonia-N, pH and ionic composition of early juvenile mud crabs, Scylla serrata (Forskål). Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 148 (2). pp. 278-285.

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Abstract

The current study was conducted to determine the LC50 value of ammonia-N as well as the effects of acute exposure to elevated ammonia on the haemolymph osmolality, ionic composition, ammonia-N and pH levels of early juvenile mud crabs, Scylla serrata. The results show that early S. serrata juveniles have a high 96-h LC50 value of 95.35 mg/L ammonia-N (6.81 mg/L NH3-N) or 6.80 mmol/L total ammonia-N (0.486 mmol/L NH3-N). Following a 96-h exposure, the haemolymph osmolality and K+ levels of the surviving crabs remained unaltered (p > 0.05) at all ammonia-N concentrations, while the haemolymph Na+ and Ca2+ were significantly lower (p < 0.05) for the crabs exposed to 5.710 and 7.138 mmol/L ammonia-N. While the haemolymph ammonia-N levels of the crabs significantly increased (p < 0.01) with increasing external ammonia-N concentrations, the haemolymph ammonia-N of the crabs remained below the external ammonia-N concentrations. The haemolymph pH of the crabs significantly increased between 0.714 and 4.283 mmol/L total ammonia-N. However, at 5.710 mmol/L total ammonia-N the haemolymph pH dropped and was not significantly different (p > 0.05) from that of the control crabs which coincided with significantly lower (p < 0.05) haemolymph Na+ and Ca2+ levels. These physiological responses may explain the high ammonia tolerance of early S. serrata juveniles.

Item ID: 2518
Item Type: Article (Refereed Research - C1)
Keywords: ammonia toxicity; haemolymph osmolality; haemolymph ionic composition; haemolymph pH; haemolymph ammonia; mud crab juveniles; Scylla serrata
ISSN: 1531-4332
Date Deposited: 22 Jul 2009 06:31
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 60%
05 ENVIRONMENTAL SCIENCES > 0599 Other Environmental Sciences > 059999 Environmental Sciences not elsewhere classified @ 40%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830199 Fisheries - Aquaculture not elsewhere classified @ 80%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 20%
Citation Count from Web of Science Web of Science 14
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