Granulocyte–macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating

Scott, Jennifer L., Ketheesan, Natkunam, and Summers, Phillip M. (2007) Granulocyte–macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating. Reproduction, Fertility and Development, 19 (4). pp. 585-593.

[img] PDF
Restricted to Repository staff only

View at Publisher Website: http://dx.doi.org/10.1071/RD06137

Abstract

Cytokines produced in the female reproductive tract after mating may enhance reproductive success. The present study investigated the distribution of granulocyte–macrophage colony stimulating factor (GM-CSF) and interleukin (IL)-8 in tissues and luminal secretions from different sites in the reproductive tract of the ewe following oestrus and after natural mating. Fifteen ewes were mated with a ram for 1 h and their reproductive tracts collected 3, 6, 18, 24 or 48 h later. Another 15 ewes were used as oestrous controls. Luminal secretions and tissue samples were collected from seven sites in each reproductive tract. Secretions were analysed by enzyme-linked immunosorbent assay and tissues were stained immunohistochemically using anti-sheep GM-CSF and anti-sheep IL-8 antibodies. Both cytokines were found in luminal and glandular endometrial epithelium and, to a lesser extent, in cervical epithelium; neither was found in the vaginal epithelium. Twice as many (P < 0.05) luminal samples from mated ewes than non-mated ewes were positive for GM-CSF. The vaginal lumen contained significantly higher (P < 0.01) concentrations of IL-8 compared with other sites, irrespective of mating status. Significant differences (P < 0.05) were found in staining intensity of GM-CSF and IL-8 from different sites. Production of GM-CSF and IL-8 by reproductive tissues is likely to contribute to leucocyte infiltration into the ovine reproductive tract.

Item ID: 2387
Item Type: Article (Refereed Research - C1)
Keywords: cytokines; ovine; semen; uterus
ISSN: 1448-5990
Date Deposited: 20 Jul 2009 05:38
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0702 Animal Production > 070206 Animal Reproduction @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830311 Sheep - Wool @ 100%
Citation Count from Web of Science Web of Science 2
Downloads: Total: 5
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page