Alzheimer's Aβ fused to green fluorescent protein induces growth stress and a heat shock response

Caine, Jo, Sankovich, Sonia, Antony, Helma, Waddington, Lynne, Macreadie, Peter, Varghese, Jose, and Macreadie, Ian (2007) Alzheimer's Aβ fused to green fluorescent protein induces growth stress and a heat shock response. FEMS Yeast Research, 7 (8). pp. 1230-1236.

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Abstract

The 42 amino acid Alzheimer's Aβ peptide is involved in the progression of Alzheimer's disease. Here we describe the effects of intracellular Aβ, produced through its attachment to either end of a green fluorescent protein, in yeast. Cells producing Aβ exhibited a lower growth yield and a heat shock response, showing that Aβ fusions promote stress in cells and supporting the notion that intracellular Aβ is a toxic molecule. These studies have relevance in understanding the role of Aβ in the death of neuronal cells, and indicate that yeast may be a new tractable model system for the screening for inhibitors of the stress caused by Aβ.

Item ID: 22328
Item Type: Article (Refereed Research - C1)
Keywords: Alzheimer's disease; protein misfolding; heat shock response; Aβ; β amyloid; reactive oxygen species
ISSN: 1567-1364
Date Deposited: 25 Jul 2012 02:26
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060111 Signal Transduction @ 25%
11 MEDICAL AND HEALTH SCIENCES > 1109 Neurosciences > 110904 Neurology and Neuromuscular Diseases @ 50%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060104 Cell Metabolism @ 25%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920112 Neurodegenerative Disorders Related to Ageing @ 100%
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